Modulation of muscle phosphofructokinase at physiological concentration of enzyme.
نویسندگان
چکیده
منابع مشابه
Fish Muscle Phosphofructokinase: Influences of Protein Concentration on Enzyme Kinetic Behaviour
Phosphofructokinase (PFK) is regulated by multiple mechanisms, one of these being changes in the polymerization state of the enzyme which is influenced by enzyme concentration. To better understand the regulation of PFK in vivo, the present study monitored the effects of changing enzyme concentration and of protein crowding on enzyme activity and properties. Purified PFK from white muscle of ra...
متن کاملEnzyme kinetics at high enzyme concentration.
We re-visit previous analyses of the classical Michaelis-Menten substrate-enzyme reaction and, with the aid of the reverse quasi-steady-state assumption, we challenge the approximation d[C]/dt approximately 0 for the basic enzyme reaction at high enzyme concentration. For the first time, an approximate solution for the concentrations of the reactants uniformly valid in time is reported. Numeric...
متن کاملModulation of the Kinetic Properties of Phosphofructokinase
Ammonium ions were shown to be much more efficient than potassium ions in activating rabbit skeletal muscle phosphofructokinase (EC 2.7.1.11). Under experimental conditions simulating physiological ones (pH 7.2, and inhibitory concentrations of ATP), the apparent dissociation constant of the phosphofructokinase-NH4+ complex was 0.33 mu. This molarity was shown to lie within the physiological co...
متن کاملSite-directed mutagenesis of rabbit muscle phosphofructokinase cDNA. Mutations at glutamine 200 affect the allosteric properties of the enzyme.
Full-length cDNA for rabbit muscle phosphofructokinase has been cloned and characterized (Li, J., Chen, Z., Lu, L., Byrnes, M., and Chang, S. H. (1990) Biochem. Biophys. Res. Commun. 170, 1056-1060). The 2.8-kilobase cDNA was inserted in the plasmid vector pPL2 and transformed into Escherichia coli cells deficient in endogenous phosphofructokinase activity (DF 1020). The recombinant phosphofruc...
متن کاملthe role of application of dynamic assessment approach in improvement of iranian efl writing performance at different language proficiency levels
the present study sought to investigate the role of dynamic assessment (da) in improvement of iranian efl writing performance at different language proficiency levels. to this end, after conducting the quick placement test, 60 iranian efl learners were assigned to two groups with different language proficiency levels. in both groups each participant wrote two compositions, one before and one af...
ذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1985
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)89522-9